Calling methods from a separate script Let's say you have script A that accesses a method from script B. Would it be more performant to merge script A and B together? Let's see! public Fibonacci FibonacciInstance; [RecursiveMethod] private int FibonacciRecursive(int n) { if (n <= 0) return 0; else return 1 + FibonacciRecursive(n - 1); } public override void Benchmark1() { for (int i = 0; i < 10000; i++) { FibonacciRecursive(2); } } public override void Benchmark2() { for (int i = 0; i < 10000; i++) { FibonacciInstance.FibonacciRecursive(2); } } Both methods do the same thing : Benchmark1 calls FibonacciRecursive in the same script Benchmark2 calls FibonacciRecursive from a separate script B1 : 236.120 ms  B2 : 305.513ms (1.29 times slower) So yes, calling a method from a separate script affects the performance.